摘要
目的研究Pitx2基因在斑马鱼牙齿早期发育阶段的表达。方法利用RT-PCR技术直接克隆Pitx2特异性基因片段,制作针对Pitx2的基因探针,选取斑马鱼发育早期多个时段的胚胎进行整胚原位杂交。结果成功克隆Pitx2基因,合成针对Pitx2的基因探针,获得Pitx2基因在斑马鱼早期发育阶段的表达情况。结论了解Pitx2基因在斑马鱼早期发育阶段中的表达情况。
Objective To study the expression of gene Pitx2 during the early state of dental development in zebrafish. Methods We synthesized gene probes for Pitx2 by cloning specific gene fragment of Pitx2 with RT - PCR directly, and then carried out the in situ hybridization with embryos from different stages during early dental development. Results We synthesized gene probes for Pitx2 by cloning Pitx2, and got the pictures and data of early expression of Pitx2 gene in zebrafish. Conclusion We got the expression of Pitx2 during the early stage of zebrafish successfully.
出处
《口腔医学》
CAS
2010年第3期129-132,共4页
Stomatology
基金
国家自然科学基金项目(30973715)
江苏省教育厅指导性计划(06KJD320126)
