自制中药醇提物对瘢痕疙瘩成纤维细胞Ⅰ型胶原蛋白、基质金属蛋白酶1及血管内皮细胞生长因子表达的影响

Effects of alcohol extractant from self-made traditional Chinese drug on expressions of typeⅠ collagen, matrix metalloproteinase 1 and vascular endothelial growth factor in keloid-derived fibroblasts

目的:探讨自制中药醇提物对瘢痕疙瘩成纤维细胞(KFB)Ⅰ型胶原蛋白、基质金属蛋白酶1(MMP-1)及血管内皮细胞生长因子(VEGF)表达的影响。方法:在体外培养KFB中加入500μg/ml自制中药醇提物,采用免疫细胞化学方法检测KFB中Ⅰ型胶原蛋白、MMP-1及VEGF的表达。结果:在自制中药醇提物的作用下,KFB中Ⅰ型胶原蛋白表达减弱,MMP-1表达增强,VEGF表达呈双向性改变。结论:自制中药可能通过影响KFB合成Ⅰ型胶原蛋白、MMP-1及VEGF而发挥治疗瘢痕疙瘩的作用。

Objective To study the effects of the alcohol extractant from the self-made traditional Chinese drug on the expressions of type Ⅰ collagen, matrix metalloproteinase 1 and vascular endothelial growth factor in keloid-derived fibroblasts （KFB）. Methods The alcohol extractant from the self-made traditional Chinese drug at concentration of 500μg/ml was added to the cultured KFB. Immunohistochemistry was applied to detect the expressions of type Ⅰ collagen, matrix metallo-proteinases 1 and vascular endothelial growth factor in KFB. Results After the extractant from drug taking action, the expression of type collagenⅠwas suppressed, the expression of MMP-1 was enhanced while the expression of VEGF was increased or decreased. Conclusions The self-made traditional Chinese drug may produce therapeutic effects by influencing the synthesis of typeⅠcollagen, MMP-1 and VEGF.