RP-HPLC法测定西洋参茎叶中二醇皂苷5种成分含量

Determination of 5 constituents in 20(S)-protopanaxdiol saponins from Panax quinquefolium stem leaf by RP-HPLC

目的建立反相高效液相色谱法(RP-HPLC)同时测定西洋参茎叶二醇皂苷中5个成分(人参皂苷Rb1、Rc、Rb2、Rb3、Rd)含量测定方法。方法采用高效液相色谱法。色谱柱AgilentTC-C18(4.6mm×250mm,5μm);乙腈-0.2%磷酸水溶液为流动相梯度洗脱;检测波长203nm;体积流量1.0ml/min;柱温40℃。结果5种成分均达到基线分离,线性良好。人参皂苷Rb1、Rc、Rb2、Rb3、Rd平均回收率分别为98.39%、97.36%、98.02%、97.70%、97.01%。结论该方法能快速、准确地测定西洋叁茎叶二醇皂苷5种成分的含量,而且重复性好。

Objective To establish a simultaneous method for determination of 5 constituents from Panax quinquefolium stem leaf 20（S）-protopanaxdiol saponins,Ginsenoside-Rb1,Rc,Rb2,Rb3 and Rd by RP-HPLC.MethodsPanax quinquefolium stem leaf 20（S）-protopanaxdiol saponins was provided by the Ji＇an Yisheng Pharmaceutical Stock Co.Ltd..The Agilent ODS column（5 μm,4.6 mm×250 mm）was used,and eluted with acetonitrile-0.2% phosphoric acid water in gradient mode.The UV detection wavelength was 203 nm,and the flow rate was 1.0 ml/min,with the temperature of column at 40 ℃.The accuracy,stability and repeability of this method was evaluated.The yield rate was measured.ResultsFive compounds were baseline separated,with good linearity.A standard curve was plotted.Our method was proved to have sound accuracy,stability and repeability.The yield rate was 98.39%,97.36%,98.02%,97.70% and 97.01% respectively for Ginsenoside-Rb1,Rc,Rb2,Rb3 and Rd.ConclusionOur method of determination is rapid and accurate,and can be used for controlling the quality of Panax quinquefolium 20（S）-protopanaxdiol saponins.