鲍曼不动杆菌耐药性分析及耐消毒剂基因的检测

Detection of antibiotic resistance and disinfectant-resistant gene in Acinetobacter Baumannii

目的:了解鲍曼不动杆菌的分布和耐药性以及耐消毒剂基因qacE△1-sul1存在的情况。方法:使用VITEK全自动微生物鉴定仪对鲍曼不动杆菌临床分离株进行鉴定,药敏试验采用Kirby-Bauer(KB)法,对51株多重耐药株的耐消毒剂基因进行PCR扩增。结果:2008-01～2009-11共分离出172株鲍曼不动杆菌,易感因素主要是高龄、有严重的基础疾病和侵入性操作。鲍曼不动杆菌对亚胺培南的耐药率为11.1%,对头孢菌素类敏感率很低。51株多重耐药菌中有49株耐消毒剂基因qacE△1-sul1阳性。结论:鲍曼不动杆菌对常用抗生素耐药率高,耐药范围广,耐消毒剂基因qacE△1-sul1的携带率较高。

Objective ： To determine the distribution, drug resistance and presence of disinfectantresistant gene qacE △ 1 - sull of Acinetobacter baumannii. Methods： A. baumannii were identified by VITEK-autmnated microbial identification. Antibiotic susceptive test was carried out by Kirby-bauer （KB）. The gene of qacE△1 in A. baumannii was analyzed by polymerase chain reaction （PCR） in 51 muhidrug-resistant isolates. Results： 172 strains of the A. baumannii were isolated from January 2008 to November 2009. Susceptible factors were senility, seriousness of underlying disease and invasive operation with infection of A. baumannii. The resistance rate of A. baumannii to Imipenem was 11. 1%, but the sensitive rate to the cephlospotins was very low. 49 of 51 strains were qacE△1-sull gene positive. Conclusion： The resistance rates to common antibiotic in A. baumannii are high and the resistance patterns are wide. There is high positive percentage of qacE△1-sull gene in multidrug-resistant A. baumannii.