摘要
去乙酰化酶1(sirtuin type1,SIRTl)是一个新的脂肪细胞调控因子,通过与其靶基因叉头转录因子1(the forkhead box O family1,FoxO1)相互作用,参与细胞增殖、分化、衰老、凋亡和代谢过程.利用吖啶橙(acridine orange,AO)染色、流式细胞仪、荧光定量PCR(quantitative real-timePCR,qPCR)等技术方法,研究SIRT1的抑制剂———烟酸胺(nicotinamide,NAM)处理后对鲁西黄牛皮下前体脂肪细胞(bovine subcutaneous preadipocytes,BSP)和肌内前体脂肪细胞(bovine intramuscular preadipocytes,BIP)凋亡的影响.观察了BSP和BIP的凋亡形态;比较了SIRT1基因抑制后,相关基因如FoxO1等在两种细胞之间的表达差异.结果表明,NAM对BSP和BIP细胞表现出相同的生长抑制作用,处理组的BSP和BIP细胞的凋亡率均显著高于对照组,其作用可能通过抑制SIRT1,激活FoxO1凋亡通路实现.SIRT1及其相关基因对BSP和BIP的调控存在不同途径.
Sirtuin type 1 （SIRTl）, a novel regulatory factor of adipocyte and myocyte, interacts with target the forkhead box O family 1 （FoxOl） to modulate cell proliferation and differentiation, aging, apotosis and metabolism. The effect of nicotinamide （NAM, inhibitor of SIRT1） on the apoptosis of bovine preadipocytes was investigated with Acridine Orange （AO） staining, the flow cytometry detection and quantitative real-time PCR （qPCR）. The results showed that NAM inhibited the differentiation of bovine subcutaneous preadipocytes （BSP） and bovine intramuscular preadipocytes （BIP）, and their apoptosis rates were higher than control. SIRT1 influenced the apoptosis of bovine preadipocytes by regulating FoxO1 and adipocyte marker genes. The different mechanisms of SIRT1 and its related genes exist in BSP and BIP.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2010年第3期297-303,共7页
Progress In Biochemistry and Biophysics
基金
国家高技术研究发展计划(863)(2008AA101010)
国家现代农业(奶业)产业技术体系资助项目(nycytx-02-04)~~
