鼻咽癌高癌家系成员基因组稳定与修复基因表达的研究

Expression of genes related to genome stability and DNA repair in nasopharyngeal carcinoma clustering families

目的:探讨鼻咽癌高癌家系成员基因组稳定与修复基因的表达情况。方法:在鼻咽癌高发区广东省中山市,选择鼻咽癌高癌家系成员为研究对象,以鼻咽炎及鼻咽癌患者为对照,抽提鼻咽组织RNA,合成cRNA,以基因组稳定和修复功能分类基因芯片杂交及化学发光法检查其基因的表达情况,并实时荧光定量PCR技术进行验证。结果:鼻咽癌高癌家系成员DNA损伤修复基因主要以上调表达为主,且上调幅度高,上调100倍以上的基因包括TEP1、MSH4和PMS2L1;下调的基因少且幅度低,泛素类基因多表现为下调,实时荧光定量PCR检验结果基本一致。结论:鼻咽癌高癌家系成员存在着特定的DNA损伤修复基因变化规律。

OBJECTIVE：To observe the expression of genes related to genome stability and DNA repair in the members of NPC clustering families.METHODS：In the Zhongshan City where there was high incidence of nasopharyngeal carcinoma（NPC）,the members of the NPC clustering families were chosed as the objects,and the patients of nasopharyngitis and NPC as the control group.The RNA from the nasopharyngeal tissue was isolated,and its cRNA was synthesized,The genome stability and DNA repair genes chip technique,chemiluminescent detection and real-time fluorescence quantitative technique were used to examine the genome stability and DNA repair genes in the nasopharyngeal tissues.RESULTS：More genome stability and DNA repair genes were up-regulated in the members of the NPC clustering families than in the NPC patients,and the range of up-regulated was high and the over up-regulated 100 times genes including TEP1,MSH4,PMS2L1.Fewer genome stability and DNA repair genes were down-regulated in the members of the NPC clustering families than in the NPC patients,the ubiquitin genes almost were down-regulated,and the results also could be confirmed by real-time fluorescence quantitative.CONCLUSION：There are specially expression characteristics of genome stability and DNA repair genes in the members of NPC clustering families.