透明质酸的瘤学分布与p-CLP示踪学研究

Distribution of hyaluronic acid in lung tumor and piglet cartilage link protein's tracer kinetics

目的:确定透明质酸(HA)瘤内分布,为肺肿瘤的早期诊断提供依据。方法:免疫组化定位肿瘤内HA的分布,亲和层析法从仔猪肋软骨提取猪软骨链蛋白(p-CLP),125I标记p-CLP进行体内分布代谢示踪学研究。结果:HA免疫显色提示肿瘤细胞外基质HA有较高表达,放射示踪实验示1～7h内实验组单位质量的放射性计数百分比(13.46%～29.14%)明显高于对照组(6.4%～13.70%),差异有统计学意义,P<0.01。结论:p-CLP在肺肿瘤内浓集,125I标记p-CLP体内代谢示踪能够为肺肿瘤等疾病的早期诊断提供实验依据。

OBJECTIVE：To determine the distribution of hyaluronic acid （HA） in lung tumor so as to provide an experimental evidence for early diagnosis of lung tumor.METHODS：The distribution of HA in tumor was determined by immunohistochmistry location,piglet cartilage link protein （p-CLP） was purified by affinity chromatography from piglet costal,and the tracer studies of p-CLP,which were used to detect the tissue distribution of HA indirectly,were determined in vivo by 125I-p-CLP,which was labeled with Na125^I.RESULTS：The immunohistochmistry of HA showed the up regulation of HA expression in extracellular matrix in tumor cells,and radiocactive trace experiments showed the CPM/mg tumor percentages（13.46%-29.14%） in the experimental group were significantly higher than the percentages（6.4%-13.70%） in the control group （P〈0.01）.CONCLUSIONS：The experiments indicate the more specific-binding concentration of p-CLP in lung tumor.The methods may be useful for early diagnosis of lung tumor.