磁流体热疗治疗小鼠恶性黑色素移植瘤的实验研究

Experimental study on therapeutic effect of magnetic fluid hyperthermia for melanoma of mouse

目的:探讨磁流体高温热疗对小鼠恶性黑色素瘤的治疗作用。方法:接种B16F10黑色素瘤细胞悬液于C57/BL6小鼠的背部皮下,当肿瘤长至直径为(0.8±0.1)cm时,将20mg磁流体直接注射到肿瘤内部,24h后在交变磁场下加温(50℃,10min),间隔24h,连续治疗3次,观察磁流体热疗后肿瘤体积的变化、瘤体的病理变化、对肿瘤生长及小鼠生存期的影响。结果:加温治疗后,部分(4/7)小鼠的瘤体完全消失,实验组肿瘤没有消失的小鼠平均生存(20.333±7.881)d,瘤体消失的小鼠生存>70d;组织学检查肿瘤细胞呈凋亡、坏死样改变。结论:磁流体热疗能有效的抑制小鼠恶性黑色素瘤的生长,延长荷瘤小鼠生存期,为其成为皮肤黑色素瘤的有效治疗手段提供了实验依据。

OBJECTIVE：To assess the therapeutic effect of magnetic fluid hyperthermia on B16F10 melanoma.METHODS：C57/BL6 mice were inoculated with B16F10 cells.When the tumor grew to（0.8±0.1）cm in diameter,magnetic fluid were directly injected at the center of tumor,then subjected to magnetic field for 10 min（50 ℃）every day for three days.The therapeutic effect was assessed by tumor volume and suivival time.RESULTS：After hyperthermia,some（4/7）of mice exhibited completely cure,whose survival time were more than 70 days.The suvival time of mice with tumor was（20.333±7.881）days.And histologic examinations showed the presence of coagulation necrosis and apoptosis in the treated tumor cells.CONCLUSION：Magnetic fluid hyperthermia results in inhibition of B16F10 melanoma growth and prolongs the survival time,which may provide a new approach in the treatment of melanoma.