期刊文献+

磁流体热疗治疗小鼠恶性黑色素移植瘤的实验研究 被引量:4

Experimental study on therapeutic effect of magnetic fluid hyperthermia for melanoma of mouse
原文传递
导出
摘要 目的:探讨磁流体高温热疗对小鼠恶性黑色素瘤的治疗作用。方法:接种B16F10黑色素瘤细胞悬液于C57/BL6小鼠的背部皮下,当肿瘤长至直径为(0.8±0.1)cm时,将20mg磁流体直接注射到肿瘤内部,24h后在交变磁场下加温(50℃,10min),间隔24h,连续治疗3次,观察磁流体热疗后肿瘤体积的变化、瘤体的病理变化、对肿瘤生长及小鼠生存期的影响。结果:加温治疗后,部分(4/7)小鼠的瘤体完全消失,实验组肿瘤没有消失的小鼠平均生存(20.333±7.881)d,瘤体消失的小鼠生存>70d;组织学检查肿瘤细胞呈凋亡、坏死样改变。结论:磁流体热疗能有效的抑制小鼠恶性黑色素瘤的生长,延长荷瘤小鼠生存期,为其成为皮肤黑色素瘤的有效治疗手段提供了实验依据。 OBJECTIVE:To assess the therapeutic effect of magnetic fluid hyperthermia on B16F10 melanoma.METHODS:C57/BL6 mice were inoculated with B16F10 cells.When the tumor grew to(0.8±0.1)cm in diameter,magnetic fluid were directly injected at the center of tumor,then subjected to magnetic field for 10 min(50 ℃)every day for three days.The therapeutic effect was assessed by tumor volume and suivival time.RESULTS:After hyperthermia,some(4/7)of mice exhibited completely cure,whose survival time were more than 70 days.The suvival time of mice with tumor was(20.333±7.881)days.And histologic examinations showed the presence of coagulation necrosis and apoptosis in the treated tumor cells.CONCLUSION:Magnetic fluid hyperthermia results in inhibition of B16F10 melanoma growth and prolongs the survival time,which may provide a new approach in the treatment of melanoma.
出处 《中华肿瘤防治杂志》 CAS 2010年第4期253-257,共5页 Chinese Journal of Cancer Prevention and Treatment
基金 肿瘤磁感应治疗关键技术的研发(Z07000200540-7041) 中国博士后科学基金(20090450417)
关键词 黑色素瘤 高温 诱发 正铁化合物 纳米技术 磁力学 小鼠 melanoma hyperthermia induced ferric compounds nanotechnology magnetics mice
  • 相关文献

参考文献11

  • 1Jordan A Scholz R, Wust P, et al. Effect of magnetic fluid hyperthermia (MFH) on C3H mammary carcinoma in vivo[J]. Int J Hyperthermia,1997,13(6) :587-605.
  • 2Yanase M, Shinkai M, Honda H, et al. Intracellar hyperthermia for cancer using magnetite cationic liposomes: an in vivo study[J]. Jpn J Cancer Res,1998,89(4):463-469.
  • 3Moroz P, Jones S K, Gray B N, et al. Magnetically mediated hyperthermia:current status and future directions[J]. Int J Hy perthermia ,2002,18(18) : 267-284.
  • 4Matsuoka F, Shinkai M, Honda H, et al. Hyperthermia using magnetite cationic liopsomes for hamster osteosarcoma[J]. Bio magn Res Technoh2004,2(1) :3.
  • 5Ito A,Tanaka K, Honda H, et al. Complete regression of mouse mammary careinoma with a size greater than 15mm by frequent repeated hyperthermia using magnetite nanoparticles[J]. J Biosci Bioeng,2003,96 (4) :364-369.
  • 6Hilger I, Hiergeist R, Hergt R,et al. Thermal ablation of tumors using magnetic nanoparticles:An in viva feasibility study[J]. Invest Radial, 2002,37(1):580-586.
  • 7Papisov M I, Bogdanov J, Schaffer B, et al. Colloidal magnetic resonance contrast agents: effect of particle surface on biodistribution[J]. J Magnetism Magn Mater, 1993, 122(1) :383-386.
  • 8Hajime S,Kazutaka M, Aki I,et al. Self-regulating hyperthermia induced using thermosensitive ferromagnetic material with a low Curie temperature[J]. Cancer Sci, 2008,99(4) :805-509.
  • 9张莹莹,张威,耿传营,廖遇平,唐劲天.不同热剂量局部热疗对小鼠黑色素瘤的疗效及其抗肿瘤免疫激发作用的比较研究[J].中国微创外科杂志,2009,9(3):250-253. 被引量:10
  • 10Ito A, Matsuoka F, Honda H, et al. Heat shock protein 70 gene therapy combined with hyperthermia using magnetic nanoparticles[J]. Cancer Gene Herapy, 2003,10(12) :918-925.

二级参考文献17

  • 1王莉,张伟京.肿瘤热疗与免疫治疗[J].国际肿瘤学杂志,2006,33(5):357-360. 被引量:7
  • 2胡永成,王继芳,卢世璧.肿瘤局部热疗后自然杀伤细胞活性变化及对肺转移影响的实验观察[J].中华医学杂志,1996,76(8):582-584. 被引量:14
  • 3Dewey WC. Arrhenius relationships from the molecule and cell to the clinic. Int J Hyperthermia, 1994,10:457 - 483.
  • 4Johannsen M, Thiesen B, Jordan A, et al. Magnetic Fluid hyperthermia (MFH) reduces prostate cancer growth in the orthotopic dunning R3327 rat model. Prostate,2005,64 (3) : 283 - 292.
  • 5Manjili MH, Wang XY, Park J,et al. Cancer immunotherapy: stress proteins and hyperthermia. Int J Hyperthermia,2002,18:506 - 520.
  • 6Kawai N, Ito A, Nakahara Y, et al. Anticancer effect of hyperthermia on prostate cancer mediated by magnetite cationic liposomes and immune-response induction in transplanted syngeneie rats. Prostate, 2005,64(4) :373 - 381.
  • 7Wissniowski TT, Hunsler J, Neureiter D, et al. Activation of tumorspecific T lymphoeytes by radio-frequency ablation of the VX2 hepatoma in rabbits. Cancer Res,2003,63 ( 19 ) :6496 - 6500.
  • 8Ostberg JR, Repasky EA. Emerging evidence indicates that physiologically relevant thermal stress regulates dendritic cell function. Cancer Immunol Immunother,2006,55 (3) :292 - 298.
  • 9Kida Y, Tsuji-Kawahara S, Ostapenko V, et al. Increased liver temperature efficiently augments human cellular immune response : T- cell activation and possible monocyte transloeation. Cancer Immunol Immunother,2006,55 ( 12 ) : 1459 - 1469.
  • 10Cippitelli M, Fionda C, DiBona D, et al. Hyperthermia enhances CD95-1igand gene expression in T lymphocytes. J Immunol,2005, 174 (1) : 223 -232.

共引文献9

同被引文献51

引证文献4

二级引证文献23

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部