造血干细胞移植层流室消毒方法的研究

Study on disinfection methods of laminar flow chamber for hematopoietic stem cell transplantation

目的探讨简化的造血干细胞移植百级层流室消毒方法的消毒效果和可行性。方法传统层流室消毒方法和简化消毒方法消毒后常规进行层流室空气细菌培养和物品细菌培养，比较两种消毒方法下空气和物品培养的合格率和细菌培养阳性率。结果传统消毒方法和简化消毒方法下空气和物品培养合格率均为100%，空气培养阳性率两者分别为4%和7.41%，物品培养阳性率分别为4%和1.85%，两组差异无统计学意义（P均〉0.05）。结论层流室简化消毒方法消毒效果达到卫生部要求，与传统消毒方法比较，消毒效果一致，但明显减轻护理工作强度，降低成本，增加病人舒适度。

Objective To explore the effects and feasibility of simplify disinfection method for 100 laminar flow hematopoietic stem cell transplantation rooms in order to improve the care and reduce costs. Methods There were two groups in this study. One group used routine disinfection methods while another used simplify disinfection method. Bacterial culture of air and articles were compared between two groups. Results The qualified rates of bacterial culture were 100 % in both methods. In addition, there were no significant differences in positive rates of air bacteria and article bacteria between two methods （ P 〉 0. 05 ）. Conclusions The effects of the two disinfection methods are same. Simplify disinfection method can meet disinfection requirements of the Ministry of Health, reduce the nursing care work intensity, improve efficiency, reduce costs. Overall, simplify disinfection is better than routine method.