猪CAPN1基因mRNA表达特性分析

Analysis on expression characteristic of CAPN1 gene at mRNA level in swine

为了进一步揭示CAPN1的功能,研究以野猪、大白猪及野家杂交猪为研究对象,利用相对定量RT-PCR方法研究其编码基因的表达特性。结果表明:CAPN1基因在组织中普遍表达,但表达量存在差异,并且野猪和大白猪具有不同的表达模式;在大白猪肌肉组织中表达的发育性变化为波浪型;在360日龄大白猪和野家杂交猪肌肉组织中的表达量存在显著差异。

In the present study, its expression characteristic was analyzed by semi-quantitative RT-PCR in Wild boar, Yorkshire and Crossbred pigs. The results showed that CAPN1 gene was unevenly distributed in all tissues examined, and the expression patterns in Yorkshire and Wild boar were different. The development change of CAPN1 gene expression in skeletal muscle and Yorkshire pigs was gradually increased with the highest level in 180-d-old pigs, and then significantly decreased in 270-d-old and 360-d-old pigs. The mRNA level in muscle of 360-d-old crossbred was much higher than that in Yorkshire pigs.