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乌鸡MSTN基因的3个转录子的克隆测序及序列分析

Cloning and sequencing of MSTN gene transcripton in black chicken
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摘要 以3周龄乌鸡腿肌为试验材料,提取RNA进行RT-PCR反应、TA克隆和测序,并进行序列测定。结果表明:乌鸡腿肌中存在3种不同的MSTN基因转录子,分别将其命名为cbMSTN-1、cbMSTN-2和cbMSTN-3;cbMSTN-1由1128bp组成,cbMSTN-2由985bp组成,cbMSTN-3由754bp组成;cbMSTN-2与cbMSTN-1相比缺失了374～517bp处的143bp碱基,cbMSTN-3与cbMSTN-1相比缺失了374～748bp处的374bp碱基;cbMSTN-2、cbMSTN-3与cbMSTN-1的同源性均为99%。 RT-PCR was applied in this experiment to study the expression of myostatin. It was found that there were three MSTN gene transcriptons of varied sizes in muscle of blackchicken by cloning and sequencing. Then they were named cbMSTN-1,cbMSTN-2 and cbMSTN-3. cbMSTN-1 was composed of 1 128 bases; cbMSTN-2 was composed of 985 bases; cbMSTN-3 was composed of 754 bases. Compared with cbMSTN-2 and cbMSTN-1, 143 bases were deleted from 374 to 517; compared with cbMSTN-3 and cbMSTN-1, 374 bases were deleted from 374 to 748; compared with cbMSTN-3and cbMSTN-2, 231 bases were deleted from 374 to 748. cbMSTN-1 held 99% homology with cbMSTN-2,four bases were changed. It held 99% homology with cbMSTN-3, six bases were changed; cbMSTN-2 held 99% homology with cbMSTN-3,,four bases were changed. The results provided experimental basis to study the mechanism of MSTN gene.
作者 袁亚琦 郝文博 王宏艳 张婷婷 胡兰
机构地区 沈阳农业大学畜牧兽医学院
出处 《黑龙江畜牧兽医》 CAS 北大核心 2010年第3期17-20,共4页 Heilongjiang Animal Science And veterinary Medicine
基金 辽宁省自然科学基金项目(20050302) 辽宁省教育厅科研基金项目(2007304)
关键词 MSTN基因 乌鸡 克隆 序列分析 MSTN cDNA black bone chicken cloning sequencing
分类号 S839 [农业科学—畜牧学]
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参考文献9

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二级参考文献13

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黑龙江畜牧兽医
2010年 第3期

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