4-氨基水杨酸钠结肠靶向微丸对溃疡性结肠炎大鼠免疫机制影响

Effect of 4-Amino Salicylic Acid Colon Specific Pellet on Ulcerative Colitis Rat Model of Immune Mechanism

目的观察4-氨基水杨酸钠(4-ASANa)结肠靶向微丸对溃疡性结肠炎大鼠的干预作用及其机制。方法采用三硝基苯磺酸(TNBS)法制备大鼠实验性溃疡性结肠炎模型,通过4-ASANa结肠靶向微丸干预14d,观察大鼠结肠大体形态损伤、组织学变化和白细胞介素-1B(IL-1B)、肿瘤坏死因子-α(TNF-α)、超氧化物歧化酶(SOD)、丙二醛(MDA)、髓过氧化物酶(MPO)等指标的变化。结果4-ASANa结肠靶向微丸能减轻溃疡性结肠炎的病理损伤,显著降低溃疡性结肠炎大鼠血清IL-1B和TNF-a含量,差异具有统计学意义(P<0.05),降低结肠组织中MDA和MPO含量,而提高SOD活性,差异具有统计学意义(P<0.05),对化学因素引起的结肠炎有较好的治疗作用。结论4-ASANa结肠靶向微丸对TNBS诱导的大鼠结肠炎具有治疗作用,抑制IL-1B和TNF-a的增殖和表达,减轻自由基的损害可能是作用机制之一。

OBJECTIVE To investigate effects and mechanism of sodium 4-amino salicylic acid colon localization pellet on chronic ulcerative colonitis.METHODS The rat ulcerative colonmitis model was made with TNBS.With 14 days of treatment through the pellets.The pathology of colon,IL-1B,TNF-a,superoxide dismutase（SOD） and malondialdehyde（MDA） were measured in all groups.RESULTS The sodium 4-amino salicylic acid colon localization pellet could alleviate pathologic damage of ulcer ative colonitis;debased IL-1B,TNF-a in serum,myeloperoxidase（MPO） and MDA in colon,increased SOD in colon;better to cure ulcrrative colonitis arosed by chemicals.CONCLUSION The sodium 4-amino salicylic acid colon localization pellet can effectively inhibit the inflammatory infiltration,inhibit IL-1B and TNF-a expression of the proliferation and to reduce free radical damage may be one of the mechanisms play a role.