大剂量尖吻蝮蛇凝血酶对家兔血浆纤维蛋白原的影响

Effect of Large Doses Hemocoagulase Agkistrodon Acutus on the Plasma Fibrinogen Level in Rabbits

目的探讨大剂量尖吻蝮蛇凝血酶对家兔血浆纤维蛋白原的影响,为临床应用提供参考。方法家兔耳缘静脉注射不同剂量的尖吻蝮蛇凝血酶,并于给药后不同时间检测其血浆纤维蛋白原含量。结果0.2～1.0U·kg-1剂量的尖吻蝮蛇凝血酶可导致家兔血浆纤维蛋白原明显下降(P<0.05),下降速度及下降幅度与剂量成正相关。剂量≥0.4U·kg-1时家兔血浆纤维蛋白原开始出现耗竭,剂量≥0.8U·kg-1时所有家兔血浆纤维蛋白原均出现耗竭。结论大剂量尖吻蝮蛇凝血酶具有明显降低家兔血浆纤维蛋白原的作用。

OBJECTIVE To study the effect of large doses hemocoagulase Agkistrodon acutus on the fibrinogen level in rabbit,to provide a reference for clinical application.METHODS The 30 rabbits were randomly divided into 5 groups and were injected with different doses of hemocoagulase Agkistrodon acutus respectively,the plasma fibrinogen level were examined after administration.RESULTS The hemocoagulase Agkistrodon acutus with 0.2-1.0 U·kg-1 doses resulted in the significant decrease of the plasma fibrinogen level of rabbits.The depletion of plasma fibrinogen was first occurred in individual rabbit at 0.4 U·kg-1 doses,and while the doses were increased above 0.6 U·kg-1（include 0.6 U·kg-1）.The depletion of plasma fibrinogen was occurred in all rabbits.CONCLUSIONS The large doses hemocoagulase Agkistrodon acutus have significant effects of decreasing the plasma fibrinogen level of rabbits.