葶苈子水提液对CHF大鼠利尿作用的影响

Diuretic Effect of Aqueous Extract of Tinglizi on CHF Rats

目的观察葶苈子对CHF大鼠的尿量及尿离子的影响,并探讨其可能的机制。方法采用腹主动脉缩窄法将Wistar大鼠成功建造CHF模型后,术后8周,将大鼠随机分成生理盐水组,CHF模型组,葶苈子高、中、低剂量组共5组,分别灌胃给药,连续给药4周后,代谢笼在水负荷条件下测定各组大鼠6h排尿量,并采用MPT光谱仪测定尿中Na+、K+浓度,滴定法测定Cl-浓度。结果葶苈子高、中剂量组能显著增加CHF大鼠排尿量,与模型组比较差异非常显著(P<0.01);葶苈子高、中剂量组尿Na+、Cl-排出量较模型组明显增加(P<0.01),高剂量组K+排出明显减少(P<0.05)。结论葶苈子具有显著的利尿作用,利尿作用的机理可能是通过抑制肾小管对Na+、Cl-和水的重吸收,从而促进Na+、Cl-和水的排出。

OBJECTIVE To observe the diuretic effect of Tinglizi and study its possible mechanisms.METHODS Chronic heart failure model was established by the coarctation of abdominal aorta.After 8 weeks of operation,40 CHF rats were divided into four groups randomly as follows：model group,Tinglizi groups（high-dose group,mid-dose group,low-dose group）,with 10 rats for each group,and 10 sham operation rats ware taken as control（physiological saline,2 mL）.Adopting metabolism cage gathered the urinary output of the loading rats in 6 hours,and tested the urinary contents of Na＋,K＋ by microwave plasma torch atomic emission spectrometry（MPT-AES）,tested the urinary contents of Cl-by titration method.RESULTS Compared with the model group,high-dose group and mid-dose group could significantly increase the urinary output and contents of Na＋,Cl-in the CHF rats＇ urine;and high-dose group could reduce the contents of K＋ in the CHF rats＇ urine.CONCLUSION Tinglizi manifests considerable diuretic effect.Its function mechanism had probably associated with inhibition of renal tubular to reabsorb water,sodium,and chloride,then caused an increase in water,sodium and chloride excretion.