摘要
目的研究脓毒症大鼠急性肾功能衰竭时中性粒细胞表面CD18和血清TNF—α浓度的变化以及地塞米松（DXM）对其影响。方法72只雄性SD大鼠随机数字表法分成对照组（A组）、脂多糖（LPS）模型组（B组）、DXM＋LPS治疗组（C组），各组在2、4h和6h时观察8只大鼠肾组织大体外观及病理学变化，测定血肌酐浓度的变化，中性粒细胞表面CD18的表达，以及用ELISA法检测血清TNF—α水平变化。结果B组肉眼观肾脏表面色泽暗红，可见包膜下点状、片状出血；C组肾脏表面有轻度充血、水肿；与A组比较，B组肾脏病理呈急性炎症改变，C组肾脏病变较B组明显减轻。在4、6h时点，B、C组血肌酐浓度与A组比较明显上升（P〈0．05），但在4、6h时点B组血肌酐浓度高于C组（P〈0．05）。B组中性粒细胞表面CD18表达量（平均荧光道数）和血清TNF—α浓度较A组明显升高（P〈0．05），C组较B组中性粒细胞表面CD18表达量和血清TNF—α浓度显著降低（P〈0．05）。结论DXM可以通过下调中性粒细胞表面CD18表达量和血清TNF—α浓度，抑制中性粒细胞在肾脏的浸润而保护脓毒症大鼠的肾功能。
Objective To investigate the effect of dexamethasone （DXM） on peripheral neutrophil CD18 and serum TNF- α in septic rats with renal failure. Methods Animal model of sepsis with acute renal failure was induced by intravenous injection of lipopolysaccharide （LPS） in male SD rats. Seventy-two male SD rats were randomly divided into three groups： group A （normal group, n=24）,group B （LPS group, n=24） and group C （LPS＋DXM,n=24）. The pathological changes of kidney tissue, blood creatinine concentration, serum TNF- α were examined at 2h, 4h and 6h after LPS was given. Results The pathological changes of kidney tissue in group C were milder than those in group B at 6 hours after LPS given. Blood creatinine concentrations in group B and group C were higher than those in group A at 4 and 6 h after LPS given; while those in group C were lower than in group B at 4 and 6 h after LPS given. Serum TNF-α levels and neutrophil CD18 in group C declined more markedly than those in group B at 4 and 6 h after LPS given. Conclusion Dexamethasone can protect the renal function in LPS-induced septic rats, which is likely to be related to the downregulation of neutrophil CD18 and TNF- α.
出处
《浙江医学》
CAS
2010年第3期320-322,326,共4页
Zhejiang Medical Journal
基金
温州市科技局立项课题（Y2005A178）
