摘要
目的:以ApoE基因敲除(ApoE-/-)小鼠为动脉粥样硬化(AS)模型,观察耐力训练对ApoE-/-小鼠AS形成的干预作用及NO系统的影响,以探讨耐力运动抗AS的可能机制。方法:将24只8周龄ApoE-/-小鼠随机分为AS模型组和运动干预组,每组12只,运动组在活动跑台上进行耐力训练,实验持续14周后,测定两组小鼠主动脉斑块面积及病理变化、血清NO和血脂水平以及主动脉iNOS和eNOS蛋白表达。结果:运动组小鼠主动脉斑块面积显著小于对照组(P<0.01),血管壁及内膜损伤程度较对照组减轻,血清NO浓度和主动脉eNOS蛋白表达显著高于对照组(P<0.01),i-NOS蛋白表达显著低于对照组(P<0.05),血脂水平两组间无显著差异。结论:耐力运动通过增加eNOS蛋白表达、抑制iNOS蛋白表达和提高血中NO水平而发挥抗动脉粥样硬化的作用。
Objective： The influence of endurance exercise on the function of preventing from atherosclerosis formation and effects of endurance exercise on NO system in ApoE-deficient mice were investigated to probe possible mechanisms of anti-atherosclerosis by endurance exercise. Methods： Twenty-four 8 week old ApoE-deficient mice were selected and divided into atherosclerosis model group and exercise group randomly. There were twelve mice in each group. Mice of exercise group were trained on treadmill for fourteen weeks. Followings were detected： 1） the square of atherosclerotic plaque and pathologi-cal change; 2） the serum NO and the blood lipid; 3） the protein expression of thoracic aortic iNOS and eNOS. Results： The square of aorta plaque was significantly smaller in ApoE-deficient mice of exercise group than that in mice of control group （P0.01）. The degree of lesion in aorta wall and tunica intima was lighter in ApoE-deficient mice with endurance exercise. The concentration of serum NO and protein expression of aorta eNOS were significantly increased （P0.01） in ApoE-deficient mice with endurance exercise. The protein expression of aorta iNOS was significantly decreased （P0.05） in ApoE-de - ficient mice with endurance exercise. There was no change on the blood lipid. Conclusions： The present study demonstrated that endurance exercise may contribute to its anti-atherosclerosis function by significantly increasing eNOS protein expression and blood NO level as well as inhibiting iNOS protein expression.
出处
《天津体育学院学报》
CAS
CSSCI
北大核心
2010年第2期122-124,133,共4页
Journal of Tianjin University of Sport
基金
天津市科技计划项目(项目编号:08JCYBJC06000)
