固相微萃取-气相色谱-质谱联用法分析香蕉球茎象甲虫体挥发物

Analysis of Body Volatiles of the Banana Corm Weevil,Cosmopolites sordidus (Germar) by Solid-Phase Microextraction-Gas Chromatography -Mass Spectrometry

为了探明香蕉球茎象甲虫体挥发物的各种成分,采用固相微萃取技术(SPME)提取香蕉球茎象甲的虫体挥发物,进行气质联用仪(GC-MS)分析鉴定。结果表明:香蕉球茎象甲雌虫、雄虫、雌雄虫(雌虫和雄虫等数量混合)分别鉴定出9种、8种和9种成分,各占总峰面积的61.05%,39.46%和17.43%。香蕉球茎象甲雌虫、雄虫和雌雄虫三种虫体挥发物共有成分有3,7-二甲基-1,3,6-辛三烯、α-荜澄茄油烯、(1,1-二甲基乙基)-2-甲氧基苯酚、邻苯二甲酸二乙酯、3,3',5,5'-四甲基联苯和邻苯二甲酸二异丁酯6种,而3,7-二甲基-1,3,6-辛三烯是香蕉球茎象甲雌虫、雄虫和雌雄虫三种虫体挥发物中含量最高的成分,为19.15%。香蕉球茎象甲虫体挥发物各成分种类多样,相对含量差异较大。

In order to investigate body volatiles of the banana corm weevil,Cosmopolites sordidus （Germar）, solid-phase microextraction （SPME） was applied to trap body volatiles ofC. sordidus , and the trapped body volatiles were analyzed using gas chromatography-mass spectrometry （GC-MS）. The results showed that： there were 9, 8 and 9 body volatiles ofC. sordidus females, males, females and males（females ： males = 1：1）, representing 61.05%, 39.46% and 17.43% of the total peak area, respectively. The same body volatiles were Ocimene, à-Cubebene, Phenol,（1.1-dimethylethyl）-2-methoxy-, 1,2-Benzenedicarboxylic acid diethyl ester, 3,3＇,5,5＇-Tetramethylbiphenyl, Phthalic acid,diisobutyl ester, and relative content of Ocimene （19.15%） was the highest in three body volatiles. There were all kinds of constituents, and relative contents were significantly different inC. sordidus .