影响墨兰×兔耳兰根状茎芽分化的因素

Factors Influencing on Shoot Differentiation of Rhizome of Cymbidium sinense×Cymbidium lancifolium

以墨兰(Cymbidium sinense)×兔耳兰(Cymbidium lancifolium)种间杂种根状茎为材料,系统研究了基本培养基、植物生长调节剂、有机附加物、切割方式等7种因素对兰花种间杂种根状茎芽分化的影响。结果表明:基本培养基对芽分化影响显著,1/2MS基本培养的平均芽分化率最高。植物生长调节剂对芽诱导率影响不显著,但对平均芽分化率影响显著,在含6-BA2.0mg/L+NAA0.2mg/L的培养基上,平均芽分化率最高,为130.00%。有机附加物对根状茎芽分化有促进作用,添加100mL/L椰子汁显著提高平均芽分化率。活性炭显著抑制根状茎芽分化。30g/L麦芽糖对芽分化有显著的促进作用。切割方式对芽分化影响显著,掰断效果最好,不切割次之,切成1cm最差。光照强度对根状茎芽分化影响显著,适宜的光照强度为500lx。

Rhizomes derived from hybrid seeds of Cymbidium sinense× Cymbidium lancifolium were employed to investigate the effects of basic medium, plant growth regulator, organic additives, activated carbon, carbon sources, cutting way and light intensity on shoot differentiation influencing of rhizome. The results showed that the basic medium had significant effect on shoot differentiation, the highest mean shoot differentiation rate were obtained by using 1/2MS as basic medium. The plant growth regulator had no significant effect on shoot induction rate, but significantly affected mean shoot differentiation rate, the highest mean shoot differentiation rate, 130.00%, appeared when 2.0 mg/L 6-BA and 0.2 mg/L NAA adopted. The organic additives and 30 g/L maltose promoted the shoot differentiation of the rhizome, and supplement of 100 mL/L coconut water in medium significantly improved mean shoot differentiation rate. Activated carbon significantly inhibited shoot differentiation. Cutting way significantly influenced shoot differentiation, the best results were achieved by separation branches from the mother rhizome followed by without cutting, the results of cutting the rhizome into segments about 1 cm in length were the worst. The effect of light intensity on shoot differentiation of rhizome was significant, and the optimum light intensity was 500 1x.