野生平榛多糖对小鼠免疫活性的影响

Effect of Wild-Hazel Polysaccharide on Immunity Activity of Mice

为测量野生平榛中多糖含量并研究其免疫活性,笔者用苯酚-硫酸法测定从野生平榛的叶片、种仁及叶片的愈伤组织中提取的粗多糖含量。以上述各多糖液(12.5mg/mL)给小鼠灌胃,10天后对小鼠的免疫器官、腹腔巨噬细胞吞噬功能及血清溶血素含量进行测定,评价多糖的免疫生物活性。结果表明,榛叶多糖含量最高,高达2.93%±0.06%,愈伤组织多糖次之为2.26%±0.01%,而榛仁多糖含量最低仅为0.40%±0.08%;榛仁多糖的免疫活性最强,愈伤组织多糖的免疫活性次之,榛叶多糖不具有免疫活性。

It could provide experimental evidence for future research and development of Hazel polysaccharide by measuring the content of polysaccharide from wild-hazel and studying its immunity activity. The content of polysaccharide from wild-Hazel leaves（PHI）, wild-Hazelnut （PHn） and wild-Hazel leaves＇ callus（PHe） were determined by phenol sulfonic speetrophotometry. Mice received respectively with PH1, PHn, Phe （12.5 mg/ mL） by gavage for ten days. After that, immune organ, the function of macrophages phagocytosis and the content of serolysin in mice were determined and their immunological activities were evaluated. The results showed, the average content of PHI was 2.93% ±0.06%, which was the highest, Phc was the second, its content was 2.26%± 0.01%, PHn content was the lowest, only 0.40%± 0.08%. Among the three polysaccharide, PHn was the strongest on the immunological activity; the second was PHc; PHI had no remarkable effect on immunity.