高效液相色谱-质谱联用法测定人血浆中兰索拉唑的浓度

Determination of Lansoprazole in Human Plasma by LC-MS/MS

目的:建立高效液相色谱-质谱联用法测定人血浆中兰索拉唑的浓度。方法:血浆样品经处理后,采用Waters AtlantisC_(18)色谱柱(100 mm×2.1 mm,3μm),流动相为甲醇-0.01%甲酸溶液(70:30),以电喷雾电离源(ESI)正离子检测,奥美拉唑为内标。结果:兰索拉唑血浆质量浓度的线性范围为5.0～1 500.0 ng·ml^(-1)(r=0.999 8),,提取回收率为78.2%～88.9%(n=5),方法回收率为90.3%～101.9%(n=5),日内和日间RSD均小于10%。结论:本法专属性强,样品处理方便,灵敏度高,适用于兰索拉唑药动学研究。

Objective： To establish an LC-MS/MS method for the determination of lansoprazole in the human plasma. Method： The lansoprazole was deproteined from plasma and the Waters Atlantis C18（100 mm x 2. 1 mm,3 μ m） column was used. The mobile phase consisted of methanol-0.01% formic acid solution（70： 30）. Eleetrospray ionization （ESI） source was applied and operated in the positive mode. Omeprazole was used as the internal standard. Result： The calibration curve was linear within 5.0-1 500.0 ng. ml-1 （ r = 0.999 8 ）. The extraction recovery was within 78.2% -88.9% （ n = 5 ） and the method recovery was within 90. 3% -101.9% （ n = 5 ）. The relative standard deviation of within day and between-day were less than 10%. Conclusion： The method is proved to be ro- bust, convenient, sensitive and suitable for the clinical investigation of lansoprazole pharmacokinetics.