HSF1基因在空间学习记忆能力中的作用研究

Role of HSF1 in spatial learning task

目的探讨热休克因子1（heatshockfactor1，HSFI）在小鼠Morris水迷宫空间学习任务中的作用。方法HSFl＋／十小鼠12只，HSFI-／-小鼠12只，进入空间学习任务实验。小鼠在Morris水迷宫中训练7d后，处死，剥离前额叶，液氮冻存，Western blots检测磷酸化的细胞外调节蛋白激酶（phospho--Extracellular regulated proteinkinases，P—Erk1／2）表达水平。结果HSFI-／-小鼠在Morris水迷宫定向航行实验中，第1天至第7天逃避潜伏期均明显长于HSF1＋／＋小鼠（P〈0．05）。Western blots显示：HSFI-／-小鼠在Morris水迷宫学习任务中前额叶内P—Erk1／2水平明显低于HSF1＋／＋小鼠（P〈0．05）相应的脑区。结论HSF1在Morris水迷宫学习任务中能够维持前额叶Erk1／2激活，因而在Morris水迷宫空间学习任务中起着重要的作用。

Objective To investigate the role of heat shock factor 1 （HSF1） in spatial learning task using mice model. Methods HSF1 -/- mice （ n ： 12） and HSF1 ＋/＋ mice （ n = 12） were trained in Morris water maze for 7 days. 90 rain after the last training episode, mice were killed, prefrontal cortex removed and frozen in liquid nitrogen and stored for determination of p- Erk1/2 expression. Results The escape latency of HSF1-/- mice was prolonged significantly in place navigation ex periment in Morris water maze compared to HSF1＋/＋ mice （P 〈 0.05）. The p--Erk1/2 expression in the prefrontal cortex was remarkably elevated in HSF1 ＋/＋ mice compared to HSF1-/- mice （P 0.05）. Conclusions HSF1 may play a role in maintaining the activation of Erk1/2 signaling in prefrontal cortex, and that may contribute significantly to spatial learning task.