GnRHⅡ蛋白在子宫内膜异位症患者中的表达及意义

Expression of GnRHⅡ protein in patients with endometriosis and its significance

目的:检测GnRHⅡ蛋白在子宫内膜异位症患者异位子宫内膜、在位子宫内膜和正常子宫内膜中的表达情况,同时分析其表达是否与子宫内膜月经周期有关。方法:采用免疫组织化学SP法检测GnRHⅡ蛋白在异位内膜、在位内膜及正常子宫内膜组织中的表达情况,并分析和比较其表达是否有差异。结果:GnRHⅡ蛋白在子宫内膜异位症患者异位、在位子宫内膜及正常子宫内膜中均有表达,阳性表达定位于子宫内膜腺体及间质细胞的细胞质;GnRHⅡ蛋白在异位内膜、在位内膜及对照组正常内膜的表达依次增强,两两比较差异有统计学意义(P<0.05);GnRHⅡ蛋白在正常子宫内膜分泌期表达强于增生期(P<0.05),且以分泌早中期最强,显著强于增生期和分泌晚期(P<0.01),而异位组或在位组的分泌期与增生期比较,差异无统计学意义(P>0.05)。结论:GnRHⅡ蛋白在子宫内膜异位症的发病中以及在人类月经生理方面可能起重要作用。

Objective To examine expression of GnRHⅡ protein in patients with-and without-endometriosis and to determine its relation to the menstrual cycle.Methods The expression of GnRHⅡ protein was detected in the normal,ectopic,eutopic endometrial tissues in women with-and without-endometriosis via immunohistochemistry SP method.Results GnRHⅡ was located in the cytoplasm of endometrial stromal cells and gland cells in all specimens.The expression of GnRHⅡ was lower in the ectopic endometrium than that in eutopic endometrium of patients with endometriosis（P〈0.05）,and it was lower in the eutopic and ectopic endometrium of patients with endometriosis than that in the endometrium of women without endometriosis（P〈0.05）.The expression of GnRHⅡ was obviously higher in the early and middle secretory phase than that in the hyperplasia phase in normal endometrium（P〈0.05）.However,there was no significant difference between the eutopic and ectopic endometrium in patients with endometriosis in the menstrual cycle（P〉0.05）.Conclusion The expression of GnRHⅡ in the ectopic and eutopic endometrium may play a crucial role in the pathogenesis of endometriosis and human menstrual physiology.