急性冠脉综合征单核细胞PPARδ表达及其与血清MMP-9和TIMP-1的相关性

PPARδ Expression in Acute Coronary Syndromes Monocyte and Its Correlation with MMP-9 and TIMP-1 in Serum

目的:观察急性冠脉综合征(ACS)患者外周血单核细胞中过氧化物酶体增殖激活受体δ(PPARδ)的表达及其与基质金属蛋白酶-9(MMP-9)、组织金属蛋白酶抑制物-1(TIMP-1)的相关性。方法:纳入52例ACS患者和46例健康对照者,分离其外周血单核细胞,采用逆转录聚合酶链反应(RT-PCR)方法检测PPARδ的表达;采用酶联免疫吸附试验检测血清MMP-9和TIMP-1的水平。结果:ACS患者外周血单核细胞中PPARδ的表达水平显著低于健康对照组(p<0.01);ACS患者血清MMP-9水平高于健康对照组(p<0.01),而TIMP-1水平低于健康对照组(p<0.01);PPARδ表达水平与MMP-9呈负相关(r=-0.421,p<0.05),而与TIMP-1呈正相关(r=0.531,p<0.05)。结论:PPARδ可能在ACS的发病中具有重要作用。

Objectives：To investigate the level of peroxisome proliferator-activated receptors δ（PPARδ） expression in peripheral blood monocytes from patients with acute coronary syndrome（ACS） and to analysis the correlation among levels of PPARδ,matrix metalloproteinase-9（MMP-9） and Tissue-Inhibitor of Metalloproteinase-1（TIMP-1）.Methods：Peripheral blood monocytes were collected from 52 patients with ACS and 46 healthy controls.The PPARδ expression was detected by RT-PCR,while the serum level of MMP-9 and TIMP-1 were measured by ELISA assay.Results：The PPARδ expression level was markedly reduced in peripheral blood monocytes from patients with ACS compared with healthy controls（p0.01）.The serum MMP-9 level was significant higher while the TIMP-1 level was obvious lower in patients with ACS than controls（both p0.01）.Furthermore,the PPAR δ expression level was negatively correlated to MMP-9 level（r=-0.421,p0.05） and positively related to TIMP-1 level（r=0.531,p0.05）.Conclusion：The PPARδ might play a pivotal role in the pathogenesis of ACS.