烟酸对HepG2细胞载脂蛋白M表达的影响及其机制

Effects and Mechanism of Niacin on the Expression of Apolipoprotein M

目的:探讨烟酸对HepG2细胞载脂蛋白M表达的影响及其机制。方法:分别以不同浓度的烟酸(0、0.25、0.5、1.0、2.0mmol/L)干预HepG2细胞24h。提取各组细胞总RNA和蛋白质,分别采用实时R T-PCR和Western Blot检测载脂蛋白M的mRNA和蛋白的表达。采用实时R T-PCR检测肝细胞核因子-1αmRNA的表达。结果:烟酸呈剂量依赖性上调载脂蛋白M基因和蛋白、肝细胞核因子-1α基因的表达(P<0.05)。结论:烟酸可上调载脂蛋白M表达,其机制可能是通过上调肝细胞核因子-1α来实现的。

Objective：To examine the effects and mechanism of niacin on the expression of apolipoprotein M in HepG2 cells. Methods：The HepG2 cells were incubated with different concentration of niacin（ 0,0.25,0.5,1.0,2.0mmol /L） for 24h. Total RNA and protein of HepG2 cells were extracted. The levels of apoMmRNA and protein were measured by real time RT-PCR and Western blot. The expression of hepatic nuclear factor（HNF） -1amRNA was measured by real time RT-PCR. Results：Niacin can dose-dependently increase the expression of apolipoprotein M gene,protein and HNF-1a gene. Conclusions：niacin can increase apolipoprotein M expression,The effects are possibly dependent on upregulating HNF-1amRNA expression.