生长激素-海藻酸钠-壳聚糖微胶囊促进骨折愈合的组织学研究

Study on Growth Hormone-Chitosan-Alginate Microcapsules in Promoting Fracture Healing

目的:观察生长激素-海藻酸钠-壳聚糖微胶囊促进兔挠骨骨折愈合的作用。方法:实验将新西兰兔80只,在制备新西兰兔右桡骨中段3mm骨缺损模型的基础上,随机分成四组:口服生长激素-海藻酸钠-壳聚糖微胶囊组、皮下注射生长激素组、口服空微胶囊组和生理盐水对照组。实验组口服生长激素-海藻酸钠-壳聚糖微胶囊和皮下注射生长激素,对照组口服空微胶囊。并于术后9、17、30、42d定期HE染色和地衣红染色观察各组的骨折愈合情况。结果:本实验HE染色结果表明,由于在骨缺损部位成纤维细胞产生的大量胶原纤维为基质,形成透明软骨及成骨细胞,骨小梁生长的基础,连接骨痂形成和骨髓腔贯通。而观察到生长激素微胶囊组各期提前生长及改建提前的形态。地衣红染色图像结果分析及直方图的分析表明:生长激素微胶囊组胶原纤维产生促进骨小梁提前形成,进而骨折处骨性骨痂的提前愈合和髓腔的提前贯通。结论:生长激素-海藻酸钠-壳聚糖微胶囊口服能促进骨折修复愈合。

Objective：To study the effects of GH-Chitosan-Alginate Microcapsules on fracture healing in Newzealand rabbits. Method：Eighty healthy Newzealand rabbits were made into models with 3mm bone defect in middle segment of radius. They were randomly divided into four groups：GH-Chitosan-Alginate microcapsules group treated orally with GH-Chitosan-Alginate microcapsules,the GH injection group injected with GH,the blank microcapsules group treated orally with blank microcapsules,the control group treated orally with NS. X-ray study was used at regular intervals after operation to observe the bone repair process. Results：The bone repair effects of GH-Chitosan-Alginate Microcapsule group were better than the orther groups 9 and 17 days after operation,and showed still better after 30 and 42 days. Conclusion：The GH-Chitosan-Alginate Microcapsules could promote fracture healing.