摘要
目的:建立北青龙衣质量控制的方法。方法:采用薄层色谱进行鉴别,展开条件为三氯甲烷-甲醇(8:2),采用高效液相色谱进行含量测定,以乙腈-水-磷酸(10:90:0.2)为流动相,指标成分为(4S)-4,5-二羟基-α-四氢萘酮-4-O-β-D-吡喃葡糖苷。结果:方法灵敏、可靠、准确、重复性好。结论:该方法可作为北青龙衣质量控制的方法。
Objective:To establish the method of quality control of the fresh rejuvenated fruits of J. mandshurica. Methods:The identification was achieved by TLC and the solvent was CHCl3-MeOH(8:2) . The content determination was achieved by HPLC and elution condition was MeCN-H2O-H3PO4(10:90:0.2) . The marker component was(4S) -4,5-dihydroxy-tetralone 4-O-D-glucopyranoside. Results:The method is sensitive,reliable,accurate and repeatability. Conclusions:It can be used for quality control of the fresh rejuvenated fruits of J. mandshurica.
出处
《现代生物医学进展》
CAS
2010年第2期348-349,共2页
Progress in Modern Biomedicine
基金
黑龙江省科技厅青年科技创新基金(QC07C65)
黑龙江省教育厅青年基金(11531279)
哈尔滨市科技局科技创新人才研究专项基金(2008RFQXS106)
关键词
北青龙衣
薄层色谱
含量测定
质量控制
the fresh rejuvenated fruits of J. mandshurica.
TLC
HPLC
quality control