自组装海藻酸钠纳米粒在正常小鼠体内分布的研究

Study on the distribution of self-assembled sodium alginate nanoparticles in mice

目的:研究自组装海藻酸纳米粒(sSAN)在小鼠的体内分布情况,探讨sSAN作为维生素D3药物载体的可行性。方法:用异硫氰基荧光素(FITC)标记sSAN和负载维生素D3的海藻酸纳米粒(sSAN-VD3),将两种标记好的纳米粒分别给予小鼠灌胃,在不同的时间将小鼠处死,分别取血清和肝、肺、肾、脾,各脏器经匀浆后,用荧光分光光度计测定其荧光强度,计算血清和各组织中sSAN和sSAN-VD3的含量。结果:经灌胃给药后在小鼠血清、肝、肾、肺中均检测到上述药物,而脾中没有检测到。给药后0.5h和1h,sSAN-VD3-FITC及sSAN-FITC在肝、肺和血清中的含量持续增加,以1h时达峰值浓度,给药2h、4h后,两种制剂的浓度逐渐降低。在肾脏中的含量随着时间的延长逐渐增加,于2h时达峰值浓度,随后逐渐降低。结论:sSAN及sSAN-VD3经小鼠灌胃给药后均可吸收入血,而且口服吸收后在肝、肺、肾和血清中均有一定的分布。

Objective：To study on the distribution of self-assembled sodium alginate nanoparticles in mice,to discuss the possibility of sSAN being drug carrier of Vitamin D3. Method：sSAN and sSAN-VD3 were labeled with FITC. The rats were intragastric administered by sSAN-FITC and sSAN-VD3-FITC and were executed at different times, and serum, liver, kidney, spleen, lung were made into tissue homogenates, determined fluorescence intensity of serum and tissue homogenates. Results：sSAN and sSAN-VD3 could be detected in serum, live, kidney and lung at different times, but not in spleen. sSAN-VD3-FITC and sSAN-FITC increased continuously in liver lung and serum after administered and reached max concentration at 1h. sSAN-VD3-FITC and sSAN-FITC increased gradually in kidney, and reached max concentration at 2h, then decrease gradually. Conclusions：Both of sSAN and sSAN-VD3 could be absorbed into the blood after intragastric administration, and could distribute in the liver, lung, kidney and serum.