细胞内铜/锌超氧化物歧化酶在人胸主动脉夹层的表达研究

Expression of intracellular copper zinc superoxide dismutase in human thoracic aortic dissection

目的:探讨细胞内铜/锌超氧化物岐化酶(copper zinc superoxide dismutase,Cu/Zn-SOD,SOD-1)在人胸主动脉夹层(humanthoracic aortic dissection,hTAD)中的表达情况及其在hTAD中的可能作用。方法:蛋白质印迹法(Western blot,WB)检测SOD-1在TAD和正常人胸主动脉(NA)中膜组织中的表达情况,免疫组织化学染色(immunohistochemistry,IHC)验证SOD-1在动脉壁中的表达和定位。结果:蛋白质印迹和免疫组化染色均显示SOD-1在TAD组表达量较NA组减低(P<0.05);免疫组化染色进一步显示,SOD-1主要位于主动脉壁中膜平滑肌细胞的胞质内,其在夹层主动脉壁中膜撕开处表达缺失。结论:SOD-1在TAD中表达量减少,可能由于参与氧化应激引起的脂质过氧化和炎症反应,以及细胞外基质(extracellular matrix,ECM)的降解等机制所致。

Objective：To investigate the intracellular copper zinc superoxide dismutase （SOD-1） expression in human thoracic aortic dissection （hTAD） and its possible roles in TAD.Methods：Western blot was performed to detect the expression of SOD-1 in dissected thoracic aortas and normal controls.Immunohistochemistry was used to validate its expression and localization. Results： Western blot and immunohistochemistry showed that the SOD-1 expression decreased in TAD compared with normaI controls （P 〈0.05）.Immunohistochemistry showed further that SOD-1 mainly Iocalized in the cytoplasm of smooth muscle cells （SMCs） in the media, disappeared in the edges of the region where the media was divulsed.Conclusion：The SOD-1 expression decreased in TAD, which could attribute to some mechanisms such as participating the lipid peroxidation and Inflammatory reaction induced by oxidative stress, in addition,the degradation of extracellular matrix（ECM）.