摘要
目的:观察转染Netrin-1基因的真皮多能干细胞(dMSCs)移植对大鼠脊髓损伤的修复作用。方法:取大鼠真皮组织,分离培养真皮多能干细胞,经转染Netrin-1基因和诱导,观察细胞形态变化,免疫细胞化学方法对分化细胞进行鉴定。Wistar大鼠在L4水平制成脊髓全横断损伤模型,伤处移植大鼠真皮多能干细胞或者转染Netrin-1基因的真皮多能干细胞。对大鼠进行动物行为学(BBB)评分和对损伤脊髓进行组织学检测。结果:转染Netrin-1基因的dMSCs诱导产生的神经元样细胞占总细胞数的比例为24.45±3.73%,而单独的真皮多能干细胞诱导产生的神经元样细胞占总细胞数的比例10.50±2.13%,二者差异显著(P<0.05)。BBB评分显示转染Netrin-1基因的dMSCs移植组明显高于单纯dMSCs移植组和空白对照组(P<0.05);转染Netrin-1基因的dMSCs移植组损伤脊髓结构的修复明显优于单纯dMSCs移植组和空白对照组。结论:转染Netrin-1基因的真皮多能干细胞移植较单纯dMSCs移植对大鼠脊髓损伤有更好的治疗作用。
Objective：To observe the treatment of spinal cord injury in rats with transplantation of dermal multipotent stem cells （dMSCs） transfected with Netrin-1 gene. Methods：Dermis of neonatal rat was isolated and the dMSCs were cultured, which were transfected with Netrin-1 gene. The differentiated cells were identified by immunohistochemical technique. The spinal cord injury of adult Wistar rats was performed in L4. dMSCs transfected with or without Netrin-1 gene were injected into the injured spinal cord. The behavior of groups was assessed by BBB score and the morphologies were analyzed by immunohistochemical technique. Results The percentage of the DMSCs transfected with Netrin-1 gene differentiated into neuron-like cells was higher than that of only DMSCs group （P〈0.05）. BBB score was higher in the dMSCs transfected with Netrin-1 transplantation group than that in the only DMSCs transplantation group and control group （P〈0.05）. The structure repair of the injured spinal cord was better in that dMSCs transfected with Netrin-1 gene transplantation group than that in the only DMSCs transplantation group and control group. Conclusion：DMSCs transfected with Netrin-1 gene are more effective than only dMSCs in treating rats spinal cord injury.
出处
《现代生物医学进展》
CAS
2010年第3期432-435,共4页
Progress in Modern Biomedicine
基金
重庆市自然科学基金计划项目(CSTC
2007BB5040)
