葛根素对小鼠的通便作用及其机制研究

Laxative Effect of Puerarin and Its Mechanism in Mice

目的:研究葛根素对便秘小鼠的通便作用,探讨其通便机制。方法:采用复方地芬诺酯制造小鼠便秘模型,观察葛根素对便秘模型小鼠的首次排便时间,12h内排便粒数,粪便性状及重量的影响;采用肠段称重法,观察葛根素对大肠水分吸收的影响;采用PowerLab信号处理系统,观察葛根素对大肠平滑肌活动的影响。结果:①三个剂量组与便秘模型组相比,小鼠首次排便时间明显缩短(P<0.05),12h内粪便粒数、重量明显增加(P<0.05),并呈剂量效应。②低、中剂量组与便秘模型组相比,大肠含水量明显增加(P<0.05),高剂量组以上指标不但明显高于便秘模型组(P<0.05),而且明显高于空白对照组(P<0.05)。③与单纯给予复方地芬诺酯相比,葛根素进一步降低了小鼠大肠平滑肌收缩幅度、频率和曲线下面积,具有显著差异性(P<0.05)。结论:葛根素具有通便作用,其机制可能是葛根素增加肠道水分促肠运动的作用大于它对肠道运动的直接抑制作用。

Objective：To investigate the laxative effect of Puerarin on constipation in mice, and to explore the involved mechanism. Methods： Constipation model was established by diphenoxylate. After the constipation model were treated with Puerarin, several factors were observed after Puerarin treated constipation model, including the first defecation time. And the effects of Puerarin on water contents of large intestine and activity of isolated large intestinal smooth muscle were studied by weighing the intestine and Powerlab signal processing system respectively. Results： ① Compared with the model group, Puerarin could shorten the first defecation time and enhance two other factors such as the amount of feces within 12 hours, the weight of feces （ P〈0. 05）. ② Compared with model group, low and medium dose of Puerarin could significantly increase the water content in large intestine （ P〈0. 05）. And the above factor in high dose Puerarin group is higher than the control and model group （ P〈0.05）. ③ Puerarin could further enhance the inhibitory effect of diphenoxylate on the scope , frequency and curve of activities of intestine smooth muscle contraction. Conclusion：Puerarin can defecate feces excretion, whose possible mechanism is that the stimulatory effect by increasing water in intestine is stronger than the direct inhibitory effect on the intestinal activities.