辛伐他汀和非诺贝特对HepG2细胞载脂蛋白M表达的影响

Effects of simvastatin and fenofibrate on the expression of apolipoprotein M

目的:观察辛伐他汀和非诺贝特及两者联合对HepG2细胞载脂蛋白M表达的影响。方法:分别以不同浓度的辛伐他汀(0、1、5、10、25μmol/L)和非诺贝特(0、50、100mmol/L)及辛伐他汀(5.0μmol/L)+非诺贝特(50mmol/L)干预HepG2细胞24h。提取各组细胞总RNA和蛋白质,分别采用实时RT-PCR和WesternBlot检测apoM的mRNA和蛋白的表达。结果:辛伐他汀和非诺贝特均呈剂量依赖性上调载脂蛋白M基因和蛋白的表达(P<0.05)。联合用药比单药更能显著上调载脂蛋白M的表达(P<0.05)。结论:他汀类和贝特类药物均可上调载脂蛋白M表达,两药联合的作用更为显著。

Objective：To examine the effects of simvastatin,fenofibrate and combination of the two drugs on the expression of apolipoprotein M in HepG2 cells. Methods： The HepG2 cells were incubated with different concentration of simvastatin （0, 1, 5, 10, 25μmol /L） ,fenofibrate（ 0, 50, 100mmol /L） and simvastatin （5.0μmol /L） ＋fenofibrate（50mmol /L） for 24h, respectively . Total RNA and protein of HepG2 were extracted. The levels of apoM mRNA and protein were measured by real time RT-PCR and Western blot. Results： both of simvastatin and fenofibrate can dose-dependently increase the expression of apolipoprotein M gene and protein. Combination of statin and fibrate will obtain more effects on expression of apolipoprotein M than either agent. Conclusions： Statins and fibrates can increase apolipoprotein M expression, Combination therapy is more effective than single-drug therapy.