酵母表面展示酶技术

Technologies for Yeast Surface Display of Enzymes

酵母表面工程是利用载体蛋白将外源蛋白以活性形式锚定于酵母细胞外表面,免去了外源蛋白的纯化和固定,并且对其有稳定作用。本文综述了酵母表面展示技术的原理、步骤、优点以及目前常见的酵母表面展示酶,如淀粉水解酶、纤维素水解酶、与木糖利用相关的酶、脂肪酶、有机磷水解酶的构建及应用。

Yeast surface engineering is established by anchoring the heterogeneous proteins in its active form to the surface of yeast cell using carrier proteins,which eliminates the process for immobilization and purification of protein and improves the stability of the displayed proteins.This paper reviewed the principle,procedure,and advantages of yeast surface display technique as well as the construction and application of yeast armed by biocatalysts,such as amylolytic enzymes,cellulolytic enzymes,key enzymes in xylose utilization,lipase,organ phosphorus hydrolase.