氯过氧化物酶-聚L-赖氨酸/GC电极的电化学特性

The Electrochemical Characteristics of CPO-PLL/GC Electrode

应用电化学方法在玻碳电极上修饰聚L-赖氨酸膜,以1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐做交联剂,固定氯过氧化物酶.修饰电极的循环伏安曲线呈现一对可逆的氧化还原峰,表明聚L-赖氨酸能够很好地促进氯过氧化物酶在电极表面的直接电子传递,这是一个受吸附控制并伴随有质子转移的准可逆电子传递过程.该电极有很好的稳定性,并能显著地电催化氧的电化学还原反应.

Poly-L-lysine （PLL） was electropolymerized onto the surface of glassy carbon （GC） electrode. 1- [ （3-dimethylamino）propyl]-3-ethylcarbodiimide hydrochloride （EDC） was used as a Cross-linking agent to immobilized Chloroperoxidase （CPO） onto the PLL/GC electrode. A pair of well-defined redox peaks was observed at CPO-PLL/GC electrode, which clearly suggested that PLL can promote the direct electron transfer between the CPO and the GC electrodes. The cyclic voltammetry results also showed that the electron transfer at CPO-PLIM GC is a surface adsorption-controlled quasi-reversible process, which is accompanied with proton transfer. The CPO-PLL/GC electrode exhibited a good electrochemical stability and excellently electrocatalytie activity for electrochemical reduction of O2.