葛根多糖和黄酮综合提取工艺优化

Response Surface Methodology as An Approach for Optimization of Simultaneous Extraction of Polysaccharides and Total Flavonoids from Radix Puerariae

研究热水浸提葛根多糖和黄酮综合提取的工艺路线。在单因素试验的基础上,选定料液比、提取时间及提取温度3个因素的3个水平进行中心组合试验,建立多糖和黄酮总含量的二次回归方程,进一步优化提取工艺条件,验证模型的有效性,通过响应面分析得到优化组合条件。结果表明,提取温度、提取时间对多糖得率均有显著影响,而料液比无显著影响。当提取工艺条件为提取时间2.2h、料液比1:25.2(g/mL)和提取温度98.4℃时,水提葛根多糖黄酮提取量达到极大值。此条件下多糖和黄酮总提取量预测值为29.0591%,验证值为28.9472%。

Radix Puerariae was used as raw material to simultaneously extract polysaccharides and total flavonoids by hot water extraction.Based on single factor experiments,a central composite design（CCD） involving three variables such as material/liquid ratio and extraction time and temperature at three levels was employed for establishing a quadratic regression model describing weighted extraction yield（at 1：1 weight ratio） of polysaccharides and total flavonoids at different levels of the above variables.The optimal values of these variables were determined by response surface analysis.Moreover,the validity of the established model was verified.Results showed that both extraction temperature and time had a significant effect on polysaccharide yield,whereas no significant effect of material/liquid ratio was observed.The predicted and observed values of weighted extraction yield of polysaccharides and total flavonoids were achieved under the following optimal conditions：extraction for 2.2 h at a material/liquid ratio of 1：25.2 and 98.4 ℃.