摘要
目的:探讨橙皮苷在碱性有机溶剂辅助下的酸促水解及产物的初步分离,以期通过此法来制备两种水解产物橙皮素-7-葡萄糖苷和橙皮素。方法:对影响水解的主要因素:酸的种类、水解方式、酸浓度、有机溶剂的种类和水解时间进行探讨。结果:水解的较佳条件为:橙皮苷先用氢氧化钠溶液和异丙醇组成的碱性有机溶剂溶解,再在100℃条件下酸水解,盐酸浓度为1.5mol/L,水解时间为15～45min。利用混合产物中3种物质在丙酮及其水溶液中溶解性的差异来分离纯化水解产物也取得了一定的效果。结论:橙皮苷在碱性有机溶剂辅助和一定的盐酸浓度下可以得到较好地水解。
To provide a method for preparing two hesperidin hydrolysates,namely hesperetin-7-glucoside and hesperetin,acid-catalyzed hydrolysis of hesperidin in an alkaline organic solvent system was explored and the resulting hesperidin hydrolysates were preliminarily separated.Crucial factors affecting acid-catalyzed hydrolysis,such as acid kind,hydrolysis mode,acid concentration,composition of alkaline organic solvent system and hydrolysis time were investigated.Results showed that full dissolution of hesperidin in a mixed system of sodium hydroxide solution and isopropyl alcohol under preheating condition followed by hydrolysis for 15-45 min catalyzed with 1.5 mol/L hydrochloric acid resulted in an optimal hesperidin hydrolysis.Moreover,a satisfying separation of 3 hydrolysis products obtained was achieved using their difference of dissolubility in acetone and water.Thus,some concentration of hydrochloric acid in an appropriate alkaline organic solvent medium can give a good catalysis for hesperidin hydrolysis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第6期60-64,共5页
Food Science
基金
浙江省重大农业科技专项(2008C02005-2)
浙江省农业科学院科技创新能力提升工程项目(2007R15Y01E01)
关键词
橙皮苷
酸水解
橙皮素-7-葡萄糖苷
分离
hesperidin
acid-catalyzed hydrolysis
hesperetin-7-glucoside
separation
