八角和八角残渣总黄酮提取工艺优化

Extraction of Total Flavonoids from Star Anise and Its Residue Left after Volatile Oil Extraction

目的:确定八角和八角残渣中总黄酮的最佳提取工艺。方法:采用乙醇热回流法提取八角和八角残渣中的黄酮类化合物,通过单因素试验和正交试验,考察乙醇体积分数、料液比、提取时间、提取次数对八角和八角残渣中总黄酮提取率的影响。结果:八角总黄酮提取最佳条件为:80%乙醇、料液比1:30、提取时间120min、提取次数3次。八角残渣总黄酮最佳提取条件为:乙醇体积分数50%、料液比为1:10、提取时间120min、提取次数3次。结论:在最佳工艺条件下,八角总黄酮提取率为23.61%,八角残渣总黄酮提取率为16.81%。

In the present study,hot ethanol reflux extraction was adopted for extracting total flavonoids from star anise or its residue left after volatile oil extraction.The optimal processes for extracting total flavonoids from two materials were investigated by single factor and orthogonal array methods.Results showed that three-time extraction for 120 min each time gave an optimal extraction of total flavonoids from star anise at a material/liquid ratio of 1：30 with 80% ethanol as extraction solvent and from star anise residue at a material/liquid ratio of 1：10 with 50% ethanol,respectively.Under the above conditions,the yields of total flavonoids from star anise and its residue were 23.61% and 16.81%,respectively.