大孔吸附树脂分离纯化峨嵋岩白菜叶总黄酮

Macroporous Resin Adsorption for Purification of Total Flavonoids from Bergenia emeiensis CY Wu Leaves

目的:筛选出分离纯化峨嵋岩白菜叶总黄酮的最佳大孔树脂型号及工艺条件。方法:通过静态、动态相结合的方法,以黄酮吸附率、解吸率为指标,确定最佳工艺。结果:HPD-600型树脂具有最佳的吸附和洗脱参数,其最佳工艺为:粗提物水溶液上样总黄酮质量浓度为0.5～0.6mg/mL,上样液pH3～4,吸附流速2mL/min,最大上样量为3BV。吸附后先以3BV水洗去杂质,再用4BV的90%乙醇以2mL/min的速率进行洗脱,黄酮纯度和回收率分别达29%和80%以上。结论HPD-600可较好地吸附分离峨嵋岩白菜叶总黄酮,纯化后黄酮纯度提高5倍以上,且操作简单、安全、成本低廉,有较高的应用价值。

An optimal macroporous resin and its optimal technological parameters for purification of crude total flavonoids separated from Bergenia emeiensis CY Wu leaves were investigated in the present study.HPD-600 resin was chosen due to its excellent adsorption and desorption performance.Static and dynamic adsorption and desorption behaviors of HPD-600 resin were evaluated for achieving an optimal purification of total flavonoids.Results showed that the optimal purification process was that sample solution at pH 3-4 and the concentration level of 0.5-0.6 mg/mL was made to flow through HPD-600 resin column with a maximum injection volume of 3 BV and subsequently,the column was washed with 3 BV of water for the removal of impurities and eluted with 4 BV of 90% ethanol at a flow rate of 2 mL/min.More than 80% total flavonoids with a purity of more than 29% were recovered using the above process.These data demonstrate the excellence of HPD-600 resin in purifying total flavonoids from Bergenia emeiensis CY Wu leaves and the advantages of the developed method for industrial application,like simple operation,safety,and low cost.