正交试验法优选鹰嘴豆种子中鹰嘴豆芽素A的提取工艺

Orthogonal Array Design for Optimization of Extraction of Biochanin A from Chickpea Seed

目的:优选鹰嘴豆种子中鹰嘴豆芽素A的提取工艺。方法:采用正交试验法,以鹰嘴豆种子中鹰嘴豆芽素A的含量为指标进行试验。结果:优选出从鹰嘴豆种子中提取鹰嘴豆芽素A的最佳工艺:10倍于生药量的95%乙醇溶液热回流提取两次,每次1.5h。结论:该工艺可提高鹰嘴豆种子中鹰嘴豆芽素A的提取率和纯度,而且方便、安全、无毒、成本低、效率高,可在大规模生产鹰嘴豆芽素A时推广应用。另外,本研究还建立了一种新的快速、准确检测鹰嘴豆种子中鹰嘴豆芽素A的RP-HPLC方法。

Orthogonal array design was used to optimize hot ethanol reflux extraction of biochanin A from chickpea（Cicer arietinium L.） seed in this study.Results showed that an optimal compromise between biochanin A yield and purity was achieved by two-time extraction at a material/liquid ratio of 1：10 for 1.5 h each time.This extraction method,thanks to its convenience,safety,low cost and high efficiency,can be popularized for the large-scale production.Moreover,based on optimized hot ethanol reflux extraction,a RP-HPLC method was developed for rapid and accurate determination of biochanin A from chickpea seed.