发光弧菌快速检测液态奶中的三聚氰胺

Application of Luminescent Vibrio fischeri to Rapid Determination of Melamine in Liquid Milk

以发光的费氏弧菌为检测菌种,应用SKALAR TOXTRACER毒素分析仪测定液态奶中三聚氰胺对费氏弧菌的毒性效应。结果表明:发光强度的抑制率与三聚氰胺溶液质量浓度呈正相关,相关系数在0.83～0.99之间;发光强度的抑制率与液态奶中的三聚氰胺质量浓度呈负相关,相关系数在0.79～0.94之间。发光菌对液态食品中三聚氰胺等毒性物质的检测具有快速、简便、灵敏和低廉的特点,发光菌的生物毒性检测法有望在食品安全方面得到广泛应用。

A method based on the toxicity effect of melamine on luminescent bacterium Vibrio fisheri measured using Skalar ToxTracer system was developed for determining melamine in liquid milk.Results showed that there was a positive correlation between inhibitory rate of melamine against luminescent intensity of Vibrio fisheri and melamine concentration in aqueous solution with a correlation coefficient ranging within 0.83-0.99,whereas inhibitory rate of melamine against luminescent intensity of Vibrio fisheri was negatively correlated with melamine concentration in liquid milk,and the correlation coefficient was between 0.79 and 0.94.This method,thanks to the characteristics of rapidity,simplicity,sensitivity and low cost,is expected to gain an extensive application to food safety detection.