眼镜蛇毒对大鼠弓状核nNOS表达的影响

Effect of Naja naja atra Venmom on Expression of nNOS in ARC of Rats

目的探讨眼镜蛇毒对大鼠弓状核神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)表达的影响。方法采用免疫组织化学方法分别显示眼镜蛇毒组、生理盐水组和正常对照组弓状核的nNOS表达。结果与生理盐水组和正常对照组相比,眼镜蛇毒组弓状核nNOS阳性神经元数量明显减少,细胞平均灰度值明显升高(P<0.05)。结论眼镜蛇毒能够使大鼠弓状核nNOS表达降低。

Objective To investigate the effects of Naja naja atra venom on the expression of nNOS in ARC of rats. Methods The methods of immunohistochemistry technique were used to observe the expression of nNOS positive cells among Naja naja atra venom group, saline group and control group. Results The number of nNOS positive cells in ARC of Naja naja atra venom group were lower than that of saline and control groups and the celluar average op- tic density value of Naja naja atra venom group was higher than that of saline and control group（P〈 0.05）. Conclusion Naja naja atra venom could decrease the expression of nNOS in ARC of rats.