普通野生稻抗源对细菌性条斑病的抗性遗传分析

Inheritance of resistance to bacterial leaf streak in four accessions of common wild rice(Oryza rufipogon Griff.)

用感细菌性条斑病品种9311为母本,4个普通野生稻抗源DY3、DY16、DY17、DY20为父本,组配了9311/DY3、9311/DY16、9311/DY17和9311/DY204个组合的F1、F2和B1C1群体。在水稻分蘖期,用广西细菌性条斑病菌优势致病型菌株JZ28,以针刺法对各世代进行接种鉴定和遗传分析。结果表明,对DY3,F2群体抗感分离比为39∶544,卡方测验x2=0.1245<x02.05,1,符合1R∶15S的理论比例;对DY16,F2群体抗感分离比为94∶343,卡方测验x2=2.655<x02.05,1,符合1R∶3S的理论比例;对DY17,F2群体抗感分离比为41∶501,卡方测验x2=1.382<x20.05,1,符合1R∶15S的理论比例;对DY20,F2群体抗感分离比为43∶489,卡方测验x2=2.745<x02.05,1,符合1R∶15S的理论比例。结合各个组合F1和B1C1植株对细菌性条斑病表现为感至高感,据此推知DY3、DY17、DY20的抗性由2对隐性抗性基因控制;DY16的抗性由1对隐性抗性基因控制。

The F1 , B1C1 and F2 populations derived from bacterial leaf streak （Xanthomonas oryzae pv. oryzicola） susceptible variety 9311 crossed with 4 wild rice resistant accessions DY3, DY16, DY17 and DY20 were used for genetic analysis by pricking inoculation with Guangxi prevailing strain JZ28 at the tillering stage. The results showed that the segregation ratio of resistance to susceptibility was 39：544 in F2 generation from cross 9311/DY3, fitting the expected ratio of 1R： 15S （x^2 =0.124 5 〈 x^20.05,1 ）. The segregation ratios in F2 generation from 9311/DY16, 9311/DY17 and 9311/DY20 were 94： 343, 41：501 and 43： 489, fitting the expected ratio of 1R：3S （x^2 =2.655〈x^20.05,1）, 1R：15S （x^2 =1.382〈X0.05,1） and 1R：15S （x^2 =2.745〈x^2 0.05,1 ）, respectively. In addition, all plants of the F1 and B1C1 generations were susceptible or highly susceptible to bacterial leaf streak. The results of present study indicated that the resistance conferred by DY3,DY17, DY20 to bacterial leaf streak was controlled by two pairs of recessive genes, and the resistance possessed by DY20 was controlled by a single recessive gene.