万寿菊根提取物对西瓜枯萎病菌的抑菌活性成分及作用机理研究

Active antifungal component of extracts from Tagetes patula root against Fusarium oxysporum f. sp. niveum and its mechanism

本文研究了万寿菊根提取物中西瓜枯萎病菌的抑菌活性成分及其抑菌作用机理。结果表明,在4类主要化学物质中,精油类含量最高(59.8%),且抑菌效果最佳;10mg/mL精油提取物的抑制率在24、36和48h分别为62.83%,58.31%和56.30%。在西瓜枯萎病的3个主要发病期(苗期、伸蔓开花期和座果期)施用精油提取液,能有效抑制西瓜枯萎病菌,促进植株生长,同时提高西瓜植株的POD和SOD酶活性,并维持CAT活性,有效减轻了西瓜枯萎病菌对植株的毒害作用。

Bioactive components from extracts of Tagetes patula root against Fusarium oxysporum f. sp. niveum （FON） were isolated and their influence on watermelon was investigated. The results showed that the essential oil content was the highest （up to 59.8% ） , and its inhibitory effect on FON was the best, which was 62.83%, 58.51% and 56.30% at the time point of 24, 36 and 48 h, respectively. The essential oil was applied at three main infection periods of watermelon Fusarium wilt： seedling, bloom and fruit setting stage. The essential oil remarkablely inhibited the mycelial growth of FON, and promoted the growth of watermelon plant. At the same time, it enhanced the enzymatic activities of superoxide dismutase （SOD） and peroxidase （POD）, maintained catalase （CAT） activity, and reduced the harm of crude toxin of FON to watermelon plant.