乙二醇及二甲基亚砜对小鼠卵母细胞冷冻后纺锤体及孤雌激活后发育能力的影响

Effect of EG and DMSO on Spindle and Parthenogenetic Development of Matured Oocyte after Vitrification in Mice

试验采用EFS30、DFS30和EDFS30冷冻液对小鼠MⅡ期卵母细胞进行玻璃化冷冻,并对冷冻-解冻后卵母细胞存活率、纺锤体形态正常率、孤雌激活胚胎的发育能力进行研究。结果表明:利用EDFS30冷冻-解冻卵母细胞后的存活率(98.3%)显著高于EFS30组(88.2%)和DFS30组(89.5%)(P<0.05);DFS30组纺锤体形态正常率(44.9%)显著低于其他各组;而孤雌激活后EFS30、DFS30、EDFS30和对照组之间卵裂率(59.1%、56.3%、59.3%和60.0%)、囊胚率(52.0%、53.7%、61.2%和62.1%)和囊胚细胞数(49、48、50、50)均无显著性差异(P>0.05)。综上所述,3种冷冻液冷冻卵母细胞孤雌激活后均可获得较好的体外发育能力,其中EDFS30较适宜小鼠MⅡ期卵母细胞冷冻保存。

The present study was designed to cryopreserve the mouse matured oocytes with vitrification solution EFS30,DFS30 or EDFS30,and then the survival,morphology of spindle after thawing.Moreover,the development potential in vitro after parthenogenetic activation were also detected.The results indicated that the rate of survival of oocytes after thawing in EDFS30 group （98.3%） were significantly higher than that of EFS30 （88.2%） and DFS30 group （89.5%）（P〈0.05）;The rate of oocytes with normal spindle in DFS30 group （44.9%） were significantly lower than that of other groups （P〈0.05）.However,there were no difference among each groups in not only the cleavage rate,blastocyst rate and the number of blastocysts after parthenogenetic activation.In conclusion,all of the vitrification solutions EFS30,DFS30 or EDFS30 could be used for mouse oocytes cryopreservation,but EDFS30 was more suitable.