UCP基因A→G(-3826)变异与中国人2型糖尿病脂代谢、体脂含量及分布的关系

Effects of A→G(-3826) variant of the uncoupling protein (UCP) gene on lipid metabolism, body fat and its distribution in type 2 diabetic Chinese

目的观察解偶联蛋白（ＵＣＰ）基因Ａ→Ｇ（－３８２６）变异与２型糖尿病脂代谢，体脂含量及分布的关系。方法２３８例中国人（其中糖耐量正常者１３２例，２型糖尿病１０６例）及６４例白种人用ＰＣＲＲＦＬＰ检测ＵＣＰ基因Ａ→Ｇ（－３８２６）变异。体脂分布参数用核磁共振测定。结果中国人与白种人ＵＣＰ基因Ａ→Ｇ（－３８２６）变异的基因型及等位基因频率分布存在显著差异。（Ｐ值分别为２．０３×１０－６及６．８×１０－７）。“Ｇ”等位基因与２型糖尿病男性腹内脂肪（ＶＡ）增加（Ｐ＝０．０２６）、２型糖尿病女性腹部皮下脂肪（ＳＡ）减少（Ｐ＝０．０１４）、股部皮下脂肪（ＦＡ）减少（Ｐ＝０．０４２）及ＴＣ增高（Ｐ＝０．００２）有关。Ｌｏｇｉｓｔｉｃ回归分析发现：ＵＣＰ基因在２型糖尿病男性中与ＶＡ（Ｐ＝０．０１３０）及ＴＧ（Ｐ＝０．００５２）有关，在２型糖尿病女性中与ＦＡ（Ｐ＝０．００９６）有关。结论ＵＣＰ基因中“Ｇ”等位基因与２型糖尿病者腹内脂肪的绝对（表现于男性）或相对（表现于女性）的增加有关。ＵＣＰ基因或与ＵＣＰ基因Ａ→Ｇ（－３８２６）变异处于连锁不平衡的邻近基因的突变可能参与人类体脂分布的调节。

Objective To investigate into the relationship between A→G(-3826) variant of the UCP gene and lipid metabolism, body fat and its distribution in type 2 diabetic Chinese. Methods The genotypes of A→G(-3826) variant of the UCP gene were determined with PCR RFLP assay in 238 Chinese (including 132 subjects with normal glucose tolerance and 106 type 2 diabetic patients) and 64 Caucasian subjects. The parameters of regional adipose tissue distribution were also measured in these two groups by Magnetic Resonance Imaging.Results Significant differences of genotype frequency and allele frequency of A→G(-3826) variant in the UCP gene were found between the Chinese and the Caucasian. “G” allele was associated with increased visceral adipose tissue area VA in type 2 diabetic men and with decreased abdominal subcutaneous adipose tissue area SA , decreased femoral subcutaneous adipose tissue area FA , and increased TC in type 2 diabetic women. Logistic regression analysis showed that VA and TG in type 2 diabetic men, as well as FA in type 2 diabetic women were associated with genetic polymorphism of the UCP. Conclusion “G” allele of A→G(-3826) variant of the UCP gene is associated with absolute increase (men) or relative increase (women) of VA in type 2 diabetic Chinese. The A→G(-3826) variant in the UCP gene or any other nearby mutation in linkage disequilibrium may take part in the regulation of the body fat distribution.