摘要
目的制备人巨细胞病毒(HCMV)p52蛋白特异性抗原。②方法用PCR技术扩增HCMVp52蛋白IgM抗原决定簇编码区DNA片段,克隆到表达载体pBV220中,转化E.coliDH5α株,用氨苄青霉素抗性和质粒酶切图谱分析法筛选阳性克隆,经温控诱导其表达。③结果重组克隆能有效表达天然蛋白p52,ELISA检测重组p52蛋白具良好的抗原特异性。④结论通过基因工程制备的重组p52蛋白可作为HCMV血清学诊断的良好抗原。
Objective To prepare p52 protein of human cytomegalovirus by using gene engineering technique. MethodsThe fragment encoding p52 epitope of IgM was amplified by PCR technique. The amplified PCR product was inserted into the expression vect or pBV220 and in turn tranformed strain E.coli DH5α. The recombinant clone was screened by ampicillin resistance and endonuclease digestion map of plasmid. The clone was induced by temperature variation. Results The recombinant clone could effectively express p52 nature protein. ELISA showed that the recombinant p52 had good specific antigenic characterization.Conclusion The recombinat p52 prepared by gene engineering technique is a good antigen in serum diagnosis of HCMV.
出处
《青岛医学院学报》
1998年第4期237-239,共3页
Acta Academiae Medicinae Qingdao Universitatis