人原发性肝癌中p15基因第二外显子突变研究

Mutation Analysis of the p15 Gene Exon 2 in Human Primary Hepatocarcinoma *

为探讨ｐ１５抑癌基因在人原发性肝癌分子发病机理中所起的作用，采用ＰＣＲ－ＳＳＣＰ对３５例人原发性肝癌、３５例癌旁肝硬化组织以及１０例正常人白细胞中ｐ１５基因第二外显子的突变进行了初步研究。结果显示：除１例癌旁肝硬化组织中发现迁移率异常的ＳＳＣＰ区带外，其余肝癌及癌旁组织未见异常ＳＳＣＰ区带。对该例异常ＳＳＣＰ区带ＤＮＡ进行克隆和序列分析，显示为３４５ｂｐ野生型的ｐ１５基因第二外显子序列。由此提示：人原发性肝癌中ｐ１５基因第二外显子突变发生率很低或没有突变。

To investigate the role p15 gene plays in the pathogenesis of human primary hepatoc arcinoma, 35 human hepatocarcinomas, 35 cases of adjacent non cancerous liver cirrhosis and the blood cells of 10 normal human were analyzed for somatic mutation in p15 gene with PCR SSCP. One case of adjacent non cancerous liver cirrhosis showed abnormal migration single strand. In the hepatocarcinomas and in the other cases of adjacent non cancerous liver cirrhosis, no mutation was found. Cloning and sequencing of the amplified abnormal migration single strand DNA revealed that it contained a wild type exon 2 of p15 gene in 345bp length. The results indicate that the inaction of p15 gene by point mutation is a very uncommon event in human hepatocarcinoma.