狭缝印迹杂交分析癌转移抑制基因nm23在人肺癌中的表达

Slot Blot Analysis of Metastasis Suppression Gene nm23 Expression in Human Lung Cancer *

为探讨癌转移抑制基因（ｎｍ２３）在人肺癌发生、发展中的作用，采用狭缝印迹杂交技术检测了不同部位、不同性质人肺组织中的ｎｍ２３－Ｈ１和ｎｍ２３－Ｈ２基因的ｍＲＮＡ表达。结果发现，ｎｍ２３－Ｈ１和ｎｍ２３－Ｈ２基因的ｍＲＮＡ表达，都有从正常肺组织、肺良性病变组织、非癌肺组织及癌旁组织、癌灶组织、到癌转移淋巴结组织逐渐降低的趋势。其中，肺癌组织较正常肺组织的ｎｍ２３－Ｈ２基因ｍＲＮＡ表达显著降低（Ｐ＜０．０５），癌转移淋巴结组织较正常肺组织的ｎｍ２３－Ｈ１和ｎｍ２３－Ｈ２ｍＲＮＡ表达均显著降低（Ｐ＜０．０５）。肺癌组织的ｎｍ２３基因表达与淋巴结有无癌转移无明显相关关系（Ｐ＞０．０５）。提示ｎｍ２３基因表达降低可能与肺癌发生有关，未发现ｎｍ２３有癌转移抑制作用。

To investigate the role of metastasis suppression gene(nm23) in the development and progression of human lung cancer, the mRNA expressions of nm23 H1 and nm23 H2 genes in a series of pulmonary tissues collected at various sites and with different properties were studied with slot blot hybridization. According to the observations on the normal pulmonary tissues, benign lesions, para and non cancer tissues, primary cancer and metastatic lymph nodes, there was a tendency of mRNA expression reduction of nm23 H1 and nm23 H2 genes. Among them, nm23 H2 mRNA expressions in lung cancer tissues were significantly decreased when compared with normal pulmonary tissues ( P <0 05), and nm23 H1 and nm23 H2 gene expressions in metastatic lymph nodes were both reduced, compared with those in normal pulmonary tissues ( P <0 05). There was no significant correlation between nm23 gene expression and lymph node metastasis in lung cancer. The results implied that reduction of nm23 gene expression might be associated with the development of lung cancer, but no evidence of metastasis suppression by nm23 gene was revealed in this study.