摘要
为探讨碱性成纤维细胞生长因子(bFGF)在慢性低氧性肺动脉高压肺血管重建中的作用及机理,采用慢性低氧性肺动脉高压大鼠模型,用酶联免疫吸附法测定其血清bFGF含量,并用原位杂交法观察肺、心、脑、肾等器官bFGFmRNA表达的变化。结果显示:低氧组血清bFGF含量(35.9±23.5pg.ml-1)明显高于对照组(6.30±0.97pg.ml-1,P<0.005);低氧组肺小动脉bFGFmRNA表达明显增强,而心、脑、肾bFGFmRNA表达无变化。提示bFGF参与了慢性低氧性肺动脉高压肺小动脉重建的调控。
To evaluate the role of basic fibroblast growth factor (bFGF) in the development of hypoxic pulmonary hypertension, 30 wistar rats were divided into hypoxic group and control group. Rat models with chronic hypoxia induced pulmonary hypertension were established. The mean pulmonary artery pressure and the ratio of free wall of the right ventricular weight to the weight of the left ventricle plus septum were measured. The Level of bFGF in serum of rats with chronic hypoxic pulmonary hypertension was determined quantitatively by ELISA. Expresion of bFGF mRNA in lung, heart, brain and kindey of rats was observed by in situ hybridization using digoxigenin labeled oligonucleotide probe. The result showed that the mPAP, the ratio of free wall of the right ventricular weight to the weight of the left ventricle plus septum, and the concentration of bFGF in serum were 3 96±0 47kPa,0 33%±0 023%,35 9±23 5 pg.ml -1 in rats exposed to hypoxia for 3 weeks, respectively; all were significantly higher than those in control group. Exprssion of bFGF mRNA in the wall pulmonary arterioles of rats with hypoxia was stronger than that of control group, but changes of expression of bFGF mRNA in heart, brain and kindey of rats were not observed. These suggest that bFGF takes part in reconstruction of pulmonary arterioles wall in chronic hypoxic pulmonary hypertension and bFGF in serum may be one of mechanism of modulating the structure remodeling of pulmonary arterioles.
出处
《华西医科大学学报》
CSCD
1998年第4期372-375,共4页
Journal of West China University of Medical Sciences
基金
国家九五攻关基金
关键词
肺动脉高压
缺氧
BFGF
ELISA
大鼠
Hypoxic pulmonary hypertension
bFGF
Enzyme linked immunosorbent assay