摘要
目的:建立以衍生化分光光度法快速测定卡托普利片中主药含量的方法。方法:在氢氧化钠碱性条件下,采用2,4-二硝基氟苯作为衍生化试剂,与卡托普利在水浴40℃暗处反应后,以342nm为检测波长,通过紫外-可见分光光度法测定卡托普利的含量。结果:卡托普利检测浓度线性范围为1.0~14.0μg·mL-1(r=0.9999),平均回收率为99.61%,RSD=1.27%(n=9),日内、日间RSD均小于2%。结论:该方法稳定、可靠,操作简便、快速,可用于卡托普利片中主药的含量测定。
OBJECTIVE: To establish derivative spectrophotometry for the determination of the main component of Captopril tablets. METHODS: Under the alkaline condition of sodium hydroxide, 2, 4-dinitrofluorobenzene was used as derivatization reagent to react with captopril at 40℃ in the water bath. Then UV spectrophotometry was adopted to detect the content of captopril with detection wavelength set at 342 urn. RESULTS: The linear range of captopril was 1.0- 14.0μg·mL^-1(r= 0.999 9) with an average recovery of 99.61% (RSD=l.27%, n=9). The RSD of intra-day and inter-day both were less than 2%. CONCLUSION: This method is stable, reliable, rapid and simple for the determination of the main components of Captopril tablets.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第13期1226-1228,共3页
China Pharmacy
基金
国家自然科学基金资助项目(30371632)
重庆医科大学资助项目(XB200332)
关键词
卡托普利片
衍生化分光光度法
2
4-二硝基氟苯
含量测定
Captopril tablets
Derivative spectrophotometry
2,4-dinitrofluorobenzene
Content determination