摘要
目的探讨增龄对雄性SD大鼠阴茎海绵体平滑肌中P47^(phox)表达的影响。方法 4月、8月和12月龄3组SD大鼠各20只,取阴茎海绵体平滑肌,应用Real-time RT-PCR和Western印迹法检测各组阴茎海绵体平滑肌组织中P47^(phox)的基因和蛋白表达的变化。结果荧光定量RT-PCR显示P47^(phox) mRNA的表达随年龄增长逐渐增高,8月龄大鼠阴茎海绵体平滑肌P47^(phox) mRNA的表达是4月龄大鼠的1.28倍,12月龄大鼠阴茎海绵体平滑肌P47^(phox) mRNA的表达是4月龄大鼠的8.57倍。Western印迹法显示,P47^(phox)蛋白在大鼠阴茎海绵体平滑肌组织中的表达随年龄增长逐渐增高。结论随着月龄的增加,P47^(phox) mRNA和蛋白质水平在大鼠阴茎海绵体平滑肌中逐渐增高,P47^(phox)可能在增龄诱导阴茎勃起功能障碍过程中发挥重要作用。
Objective To investigate the effect of aging on the expression of P47phoxin rat penile corporal cavernosum smooth muscle. Methods Sixty male Sprague-Dawley rats were divided into 3 groups based on age （4, 8, 12 months old respectively, 20 rats in each＇group）. The penile corpora cavernosa smooth muscles of rats were collected for detecting the gene expression and protein levels of P47phox Results Aging led to an increase in P47phox mRNA and protein levels in male Sprague-Dawley rats. The P47phox mRNA level in 8-month old group was 1.28 fold higher than that in 4-month old group. The P47phox mRNA levels in 12-month old group were 8.57 fold higher than that in 4-month old group. Conclusion All the results suggest that aged rats with high levels of P47phox gene and protein expression might contribute to age-related erectile dysfunction.
出处
《中国男科学杂志》
CAS
CSCD
2010年第3期17-20,共4页
Chinese Journal of Andrology
